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Please use this identifier to cite or link to this item: http://hdl.handle.net/2075/2299

Title: Manatee blood parameter research. Final report.
Authors: Walsh, Catherine J.
Luer, Carl A.
Keywords: marine mammals
Trichechus manatus
Florida (United States)
Issue Date: 17-Jun-1998
Publisher: Mote Marine Laboratory, Sarasota, FL.
Series/Report no.: Mote Technical Report;No. 580
Abstract: During the project, studies to investigate lymphocyte proliferation in both captive and free-ranging manatees were initiated. The captive manatees were from Homossassa Springs, SeaWorld, Miami Seaquarium, and Mote Marine Laboratory, all in Florida. The wild-caught manatees were from southwest Florida. The overall purpose of the study was to establish routine methodology for consistent proliferation of lymphocytes isolated from manatee whole blood. The parameters assessed for culture conditions included testing two different T-cell mitogens, concanavalin A (ConA) and phytohemagglutinin (PHA) at varying concentrations, and one B-cell mitogen, lipopolysaccharide (LPS). Data accumulated resulted in determining the optimal mitogens, their most effective concentrations, and the window of maximal lymphocyte proliferative activity.
Description: 26 p. pdf. Includes bibliographical references, table of contents, charts and tables.
URI: http://hdl.handle.net/2075/2299
Appears in Collections:Mote Technical Reports

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